You should not need to turn the heating off just to remove the loop. There will typically be a tap at both ends (or sometimes just a tap at one end and a non return valve at the far end). Its purpose is just to add water to the system. Normally it should be completely off. The purpose of actually removing (at least one end of) the hose is just to check that the taps are not letting by. If they are, then that could explain the apparent rise in pressure.
Another possibility is the that the pressure gauge itself does not work correctly any more. (I met one of those the other day when I helped a friend remove a rad and tweak some CH pipework - when I went to re-pressurise the gauge seemed stuck on about 1/4 bar and would not budge)
Expansion chamber issues are usually when it does not handle expansion correctly - so you would expect to see the pressure shoot up when the system heats. That quite often results in the PRV letting go, and then the system may have insufficient pressure to restart the following day when starting from properly cold.
Its quite common for one rad in particular to acquire most of the air that comes out of the system. You also normally can bleed more out of it some time having fully bled it a week before - again as more dissolved air comes out of the water over time.